A Zeiss LSM 510 Pascal Inverted Confocal Microscope With 488 514 633 Lasers Carl Zeiss Inc Was Used To Capture Fluorescent Images Which Were Processed And Viewed Using LSM Image Browser Software Timelapse Imaging Of CED1GFP
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Flatmount Retinas Were Stained With AntiPECAM Antibody And The Radius Of The Vascularized Area Was Determined By Measuring The Length Between The Center Of The Optic Nerve And The Edge Of Leading Vessels LSM Image Browser
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The Images Were Processed And Quantified Using LSM Image Browser As Follows Average Pixel Intensity Of The Fluorescence In The Entire Cell Was Determined For Each Spectral Window GPS1 Transfected Cells Negative Control Showed
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Image Projections And Vertical Crosssections Xz And Yz Planes Were Generated LSM Image Browser Zeiss Pilot Studies Confirmed That Bacteria Attached To Contact Lenses Removed From Infected Corneas Retained The GFP Plasmid
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Threedimensional Projection Images Were Created Following Zseries Acquisition Using The Zeiss LSM Image Browser For Some Experiments The Styryl Dye FM143 Invitrogen Carlsbad CA Was Applied To The Mounted Tissue At 5 M For 10
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The Radius Of The Vascularized Area Was Measured As Described Previously25 Briefly The Radius Was Determined By Measuring The Length Between The Center Of The Optic Nerve And The Edges Of Leading Vessels LSM Image Browser
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Images 512 512 Pixels Were Optimized And Overlay Images YFPCFP Or YFPCFPDAPI Created With LSM Image Browser A Time Series Consisting Of Images That Were Captured Every 5 Min Was Obtained For Each Cell
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